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Studies on the Developmental Cycle of Chlamydia trachomatis: Isolation and Characterization of the Initial Bodies

Laboratory for Molecular Virology, Department of Virology, Institute of Microbiology, Hebrew University-Hadassah Medical School, Jerusalem, Israel

ABSTRACT

The initial bodies which develop in the inclusion bodies of trachoma agent (Chlamydia trachomatis) were separated from the infected cells nuclei and cytoplasmic components by zone centrifugation in sucrose gradients. The initial bodies are the site of the agent's ribonucleic acid synthesis and serve as precursors to the elementary bodies. The conversion of the initial bodies to elementary bodies is through a process which resembles binary fission. The effects of antibiotics on the development of the trachoma agent initial bodies revealed that rifampin prevented and hydroxyurea affected the formation of the initial bodies. Penicillin led to the formation of structures larger than the initial bodies.