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Methionine Limitation in Escherichia coli K-12 by Growth on the Sulfoxides of D-Methionine

Basic Science Laboratory, Veterans Administration Hospital, and Department of Biochemistry, Duke Medical Center, Durham, North Carolina 27705

ABSTRACT

The synthesis and resolution of the diastereoisomers of D-methionine sulfoxide in high yield is described. Growth of two methionine auxotrophs (strains HfrC and AB1932) on the D-methionine sulfoxides is slower than on L-methionine, and the resultant cells are markedly derepressed for three enzymes of the methionine regulon (cystathionine synthetase, cystathionase, and S-adenosyl-L-methionine synthetase). Strain HfrC grows more rapidly on the sulfoxides and shows less derepression than strain AB1932. Although growth on D-methionine-d-sulfoxide is much slower than on D-methionine-l-sulfoxide (two- to threefold increase in division times), cells grown on D-methionine-l-sulfoxide generally have higher enzyme activities. The sulfoxides of D-methionine appear to provide a useful supplement to obtain methionine-limited growth in Escherichia coli.

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