a Department of Biology, University of Chicago, Chicago, Illinois 60637
ABSTRACT
Procedures are described for obtaining efficient mating (zygote formation) in the heterothallic yeast Saccharomyces cerevisiae. The methods separate operationally the initial, mating-specific aggregation from subsequent steps in conjugation. This cell-cell interaction has been characterized. The data support the conclusions that (i) aggregation in liquid suspension is a random collision process, and (ii) only non-budded cells participate in aggregation. A mathematical model for the kinetics of aggregation in liquid suspension has been developed which is in good agreement with the experimental data.
1 Present address: Department of Genetics, University of California, Berkeley, Calif. 94720.
| Appl. Environ. Microbiol. | Infect. Immun. | Eukaryot. Cell |
|---|---|---|
| Mol. Cell. Biol. | J. Virol. | Microbiol. Mol. Biol. Rev. |
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