This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Whitfield, H. J. Articles by Levine, G. Search for Related Content PubMed PubMed Citation Articles by Whitfield, H. J. Articles by Levine, G.

 Previous Article  |  Next Article 

J Bacteriol. 1973 October; 116(1): 54-58
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Isolation and Characterization of a Mutant of Salmonella typhimurium Deficient in a Major Deoxyribonucleic Acid Polymerase Activity

^a Department of Biological Chemistry, The University of Michigan, Ann Arbor, Michigan 48104

ABSTRACT

A mutant of Salmonella typhimurium strain LT2 that is deficient in a major deoxyribonucleic acid (DNA) polymerase activity has been isolated and characterized. This mutant resembles the pol mutants of E. coli in that it has low DNA polymerase activity and it is sensitive to methyl methane sulfonate as well as ultraviolet irradiation. Revertants selected for methyl methane sulfonate resistance are no longer sensitive to ultraviolet irradiation and contain normal DNA polymerase levels. No direct role in replication can be ascribed to this polymerase activity since cells grow well in its absence. In addition, the LT2 plasmid has been shown to exist in the mutant strain.

¹ Present address: Wayne State University School of Medicine, Detroit, Mich. 48201.

This article has been cited by other articles:

• Guard-Petter, J., Parker, C. T., Asokan, K., Carlson, R. W. (1999). Clinical and Veterinary Isolates of Salmonella enterica Serovar Enteritidis Defective in Lipopolysaccharide O-Chain Polymerization. Appl. Environ. Microbiol. 65: 2195-2201 [Abstract] [Full Text]