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J Bacteriol. 1973 November; 116(2): 1064-1066
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

General Method for the Isolation of Plasmid Deoxyribonucleic Acid

Patricia Guerry, D. J. LeBlanc and Stanley Falkow

1 Department of Microbiology, School of Medicine, University of Washington, Seattle, Washington 98195

ABSTRACT

Plasmid deoxyribonucleic acid (DNA) ranging from 5 x 106 to 65 x 106 daltons may be isolated from chromosomal DNA by the preferential precipitation of the higher-molecular-weight chromosomal DNA in the presence of sodium lauryl sulfate and a high concentration of NaCl.

J Bacteriol. 1973 November; 116(2): 1064-1066
Copyright © 1973 American Society for Microbiology. All Rights Reserved.



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