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Isolation of a Specialized Lambda Transducing Bacteriophage Carrying the Beta Subunit Gene for Escherichia coli Ribonucleic Acid Polymerase

^a Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138
Department of Biochemistry, Stanford University Medical Center, Stanford, California 94305

ABSTRACT

A heat-inducible lysis-defective prophage has been integrated directly into the E. coli chromosome at a site (bfe) very closely linked to the ribonucleic acid polymerase mutation rif^d, a dominant rifampin resistance allele. This unusual lysogen has facilitated the isolation of specialized transducing phages conferring rifampin resistance to sensitive cells, and carrying at least the ß subunit gene of RNA polymerase in intact form.

¹ Present address: MRC Molecular Genetics Unit, Department of Molecular Biology, University of Edinburgh, King's Buildings, Edinburgh, Scotland EH9 3JR.

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