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J Bacteriol. 1973 November; 116(2): 535-540
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Isolation of Temperature-Sensitive Membrane Mutants of Escherichia coli K-12

Johan F. Steenbakkers, J. H. F. F. Broekman, A. Kerkenaar and P. G. De Haan

Laboratory for Microbiology, State University, Catharijnesingel 59, Utrecht, The Netherlands

ABSTRACT

Mutants with impaired biosynthesis of unsaturated fatty acids or altered metabolism of the phospholipids were isolated at a rather high frequency from a set of temperature-sensitive lysis mutants. It is suggested that preselection for the lysis phenotype makes it possible to isolate several kinds of mutants affected in the integrity of the cytoplasmic membrane.

J Bacteriol. 1973 November; 116(2): 535-540
Copyright © 1973 American Society for Microbiology. All Rights Reserved.





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Copyright © 1973 by the American Society for Microbiology. All rights reserved.