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Transport of Octanoate by Pseudomonas oleovorans

^a Department of Chemistry, Indiana University of Pennsylvania, Indiana, Pennsylvania 15701

ABSTRACT

The properties of a system for octanoate transport in Pseudomonas oleovarans are described. Transport is inducible and energy dependent, shows saturation kinetics, and concentrates against a gradient. Optimal transport is at pH 6.0 and 28 C. Apparent K_m and V_max values are, respectively, 7.0 µM octanoate and 0.68 nmol of octanoate transported per min per mg (dry mass) of cells. Fatty acids from C₇ to C₁₂ are competitive inhibitors, whereas alkanes, alkenes, and esters of the same carbon chain lengths show no inhibition. The K_i values for heptanoate, nonanoate, decanoate, undecanoate, and dodecanoate are 17, 3.4, 3.2, 1.2, and 2.4 µM, respectively. The molecular specificity of this transport system is a linear hydrocarbon chain of no less than 6 to at least 11 carbon atoms and a carboxyl group.

¹ Present address: Department of Biochemistry, School of Chemical Sciences, University of Illinois, Urbana, Ill. 61801.