This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Skogerson, L. Articles by Wakatama, E. Search for Related Content PubMed PubMed Citation Articles by Skogerson, L. Articles by Wakatama, E.

Modification of Ribosomes in Cryptopleurine-Resistant Mutants of Yeast

Department of Biochemistry, College of Physicians and Surgeons, Columbia University, New York, New York 10032
Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92664

ABSTRACT

Cryptopleurine-resistant mutants of Saccharomyces cerevisiae were isolated. A single, recessive nuclear gene, very closely linked to the mating locus (2.1 centimorgans), is responsible for resistance. Ribosomes from the mutants were found to be resistant to cryptopleurine when analyzed by poly(U)-directed polyphenylalanine synthesis. Analysis of the distribution of ribosomes between monosomes and polysomes in sensitive cells exposed to cryptopleurine suggests that some step is inhibited during the elongation phase of protein synthesis.