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J Bacteriol. 1973 December; 116(3): 1091-1095
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Identification of the Amide-Linked Fatty Acids of Acholeplasma axanthum S743 as D(–)3-Hydroxyhexadecanoate and Its Homologues

^a Department of Microbiology, University of South Dakota, Vermillion, South Dakota 57069

ABSTRACT

The amide-linked fatty acids of the sphingolipids of Acholeplasma axanthum S743 are predominantly hydroxy acids. These acids were shown by gas-liquid chromatography, mass spectrometry, and polarimetry to be the D(–)3-hydroxy fatty acids. The predominant component of the mixture was 3-hydroxyhexadecanoate (ß-hydroxypalmitate, hydroxy [h] 16:0) followed by h 20 ( = unsaturated), h14:0, h12:0, and h18 in decreasing order of concentration. The fatty acid profile indicates that these ß-hydroxy acids possibly arise from elongation of the fatty acids supplied in the growth medium.

¹ Present address: C.S.I.R.O., Animal Health Laboratory, Parkville 3052, Victoria, Australia.