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J Bacteriol. 1973 December; 116(3): 1136-1144
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Reversibility of the Specific Adsorption of Colicin E2-P9 to Cells of Colicin-Sensitive Strains of Escherichia coli

R. Shannon1 and A. J. Hedges2

Public Health Laboratory, Bristol BS2 8EL, England
Department of Bacteriology, University of Bristol, BS8 1TD, England

ABSTRACT

The adsorption of colicin E2-P9 to its specific receptors on cells of sensitive strains of Escherichia coli is reversible under normal experimental conditions. At temperatures above 20 C, colicin may desorb from one cell and be readsorbed by a second with potentially lethal consequences. However, desorption of colicin seems unable to rescue a cell once it has received a lethal dose. These findings have implications both for the nature and types of specific receptors, and for the assay of colicin by the survivor count (lethal unit) methods.


J Bacteriol. 1973 December; 116(3): 1136-1144
Copyright © 1973 American Society for Microbiology. All Rights Reserved.




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