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Temperature-Sensitive Nonsense Mutations in Essential Genes of Escherichia coli

^a Department of Microbiology, University of Michigan Medical School, Ann Arbor, Michigan 48104

ABSTRACT

Cells containing nonsense mutations in essential genes have been isolated in a strain of Escherichia coli that carried the su4^ts gene which specifies a temperature-sensitive tyrosine transfer ribonucleic acid. Such cells are unable to form colonies at temperatures which inactivate this suppressor transfer ribonucleic acid. A screening procedure for the identification of mutants that carry temperature-sensitive nonsense mutations in essential genes is described, and certain properties of two such mutants are reported.

¹ Present address: Department of Microbiology, Albany Medical College, Albany, N.Y. 12208.