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J Bacteriol. 1974 January; 117(1): 242-251
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Changes in Transfer Ribonucleic Acids Accompanying Encystment in Acanthamoeba castellanii

Janis McMillen1, M. Nazario2 and T. Jensen3

1 Department of Microbiology, Kansas University Medical Center, Kansas City, Kansas 66103
Department of Biochemistry, Kansas University Medical Center, Kansas City, Kansas 66103
3 Department of Molecular Biology, Kansas University Medical Center, Kansas City, Kansas 66103

ABSTRACT

Transfer ribonucleic acid (tRNA) from exponentially growing cells (trophozoites) and from precysts of Acanthamoeba castellanii were examined by reversed-phase column (RPC-2) chromatography. This system gave excellent resolution of isoaccepting species of tRNA. The tRNAs for 12 amino acids were studied. A comparison of trophozoite and precyst tRNA elution profiles revealed no apparent differences in the number of isoaccepting species of alanyl-, arginyl-, asparaginyl-, glycyl-, leucyl-, lysyl-, methionyl-, phenylalanyl-, tryptophanyl-, or valyl-tRNAs. Seryl-tRNAs from trophozoites were eluted as three components, whereas precyst seryl-tRNAs were eluted as only two components. Precharged trophozoite and precyst isoleucyl-tRNAs were both eluted as single components; however, post-chromatography charging of trophozoite tRNA resulted in three components of activity for tRNAIle and only one component for precyst tRNAIle. None of the observed changes could be attributed to differences in synthetases or to the presence of altered tRNA lacking the CCA terminus or partially degraded by nucleases. The possible significance of these observations is discussed.

J Bacteriol. 1974 January; 117(1): 242-251
Copyright © 1974 American Society for Microbiology. All Rights Reserved.





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