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J Bacteriol. 1974 January; 117(1): 312-314
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Phenotypic Recognition of Pyocyanine Mutants in Pseudomonas aeruginosa

Melinda Carson and Roy A. Jensen

Department of Microbiology and Immunology, Baylor College of Medicine, Houston, Texas 77025

ABSTRACT

Certain classes of pyocyanine mutants in Pseudomonas aeruginosa escape detection when screened in the presence of wild-type or other mutant cells. A technique is described for recognizing mutant phenotypes after cells are in individual agar wells. The procedure eliminates cross-feeding phenomena as well as the masking of mutant phenotypes by diffusing pyocyanine produced by nearby clones.

J Bacteriol. 1974 January; 117(1): 312-314
Copyright © 1974 American Society for Microbiology. All Rights Reserved.



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Copyright © 1974 by the American Society for Microbiology. All rights reserved.