This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Hill, C. W. Articles by Dolph, W. Search for Related Content PubMed PubMed Citation Articles by Hill, C. W. Articles by Dolph, W.

 Previous Article  |  Next Article 

J Bacteriol. 1974 February; 117(2): 351-359
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Three Different Missense Suppressor Mutations Affecting the tRNA_GGG^Gly Species of Escherichia coli

¹ Department of Biological Chemistry, The Pennsylvania State University, Hershey Medical Center, Hershey, Pennsylvania 17033

ABSTRACT

The Escherichia coli suppressor mutation, supT, has been shown to cause a C U substitution in the middle position of the tRNA_GGG^Gly anticodon. This is the same tRNA species that is altered by the glyUsu_AGA mutation studied previously. This finding indicates that the supT mutant tRNA reads the glutamic acid codon, GAG. The supT suppressor has also been converted to a new suppressor, called glyUsu_GAA, which will suppress the GAA mutation, trpA46. The in vivo suppression efficiencies of each of these three missense suppressors has been measured and are as follows: glyUsu_AGA, 3.6%; supT, 1.6%; and glyUsu_GAA, 0.4%. Mistranslation by these mutant glycine tRNA species has no adverse affects on cell growth since cultures possessing the suppressors grow as fast as cells without. The supT tRNA species can be observed as a peak in the profile of glycyl-tRNA fractionated on a RPC-5 chromatographic column, indicating that the mutant tRNA can be aminoacylated with reasonable efficiency. This finding contrasts with previous findings concerning the glyUsu_AGA mutant tRNA which is not significantly aminoacylated under the same conditions.