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J Bacteriol. 1974 February; 117(2): 373-378
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

C₄-Dicarboxylate Transport in Bacillus subtilis Studied with 3-Fluoro-L-Erythro-Malate as a Substrate

¹ Institut für Genetik, 5 Köln, 41, Weyertal 121, Federal Republic of Germany

ABSTRACT

Bacillus subtilis cells grown in yeast extract medium accumulated 3-fluoro-L-erythro-[1,2-¹⁴C₂]malate more than 30-fold from the surrounding medium. No metabolic products derived from 3-fluoro-L-erythro-malate could be detected in these cells. L-Malate competitively inhibited transport of 3-fluoro-L-erythro-malate. This malate analogue was itself a competitive inhibitor of L-malate uptake. Cells that had been grown in yeast extract supplemented with 5 mM L-malate showed a 10-fold increased affinity towards 3-fluoro-L-erythro-malate relative to cells grown in yeast extract medium with no added malate. Our results suggest that two transport systems for L-malate can be induced in B. subtilis. The first of these systems seems to effect uptake of C₄-dicarboxylates (L-malate, succinate, and fumarate) in yeast extract medium. The second transport system (or possibly a modification of the first transport system) seems to be induced by addition of L-malate to this medium and is also functioning in malate minimal medium.

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