This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Terawaki, Y. Articles by Nakaya, R. Search for Related Content PubMed PubMed Citation Articles by Terawaki, Y. Articles by Nakaya, R.

 Previous Article  |  Next Article 

J Bacteriol. 1974 February; 117(2): 687-695
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Effects of Inhibition and Restoration of Protein Synthesis on the Replication of the R Factor Rts1 in Proteus mirabilis

Department of Microbiology, Institute of Public Health, Minato-ku, Tokyo 108, Japan
Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706

ABSTRACT

The effect of inhibition of protein synthesis on the replication of the R factor Rts1 in Proteus mirabilis was examined by using the technique of CsCl density gradient centrifugation. Only 12% of the copies of Rts1 were found to replicate during amino acid starvation, whereas there was a 30% increase in the amount of P. mirabilis chromosomal deoxyribonucleic acid (DNA) during the same period. Essentially the same amount of Rts1 and host chromosome replication was observed when chloramphenicol was used to inhibit protein synthesis. The replication of Rts1 DNA was also examined in experiments in which cultures were starved for amino acids in ¹⁴N-labeled medium and then transferred to ¹⁵N-labeled medium containing the required amino acids. These experiments showed that Rts1 replication took place throughout the first generation in ¹⁵N-labeled medium and that each copy of Rts1 was replicated one time during the first generation of chromosomal DNA synthesis in ¹⁵N-medium.