This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bryant, R. E. Articles by Sypherd, P. S. Search for Related Content PubMed PubMed Citation Articles by Bryant, R. E. Articles by Sypherd, P. S.

Genetic Analysis of Cold-Sensitive Ribosome Maturation Mutants of Escherichia coli

Department of Medical Microbiology, College of Medicine, University of California, Irvine, California 92664

ABSTRACT

Four cold-sensitive mutants of Escherichia coli, which have defects in the maturation of the 50S ribosomal subunit, were isolated. Each of the mutations was shown to map at a different locus. The loci were assigned the name rim (ribosome maturation) and were shown to map as follows: rimA is co-transduced with ilvD and with pyrE; rimB is co-transduced with aroD; conjugation experiments limited rimD to a region between ilv and malB, and conjugation experiments limited rimC to the 22 to 30 min region of the chromosome. In merodiploids heterozygous for rimA, rimB, or rimD, the wild-type allele was shown to be dominant to the mutant allele. The observation that the rim loci lie outside the strA region and separate from each other, as well as the recessive character of the rim loci, suggests that the mutants may be defective in ribosome maturation factors rather than being defective in ribosomal structural proteins.

This article has been cited by other articles:

• Abe, F., Minegishi, H. (2008). Global Screening of Genes Essential for Growth in High-Pressure and Cold Environments: Searching for Basic Adaptive Strategies Using a Yeast Deletion Library. Genetics 178: 851-872 [Abstract] [Full Text]  
• Kaczanowska, M., Ryden-Aulin, M. (2007). Ribosome Biogenesis and the Translation Process in Escherichia coli. Microbiol. Mol. Biol. Rev. 71: 477-494 [Abstract] [Full Text]  
• Todaka, Y., Wang, Y., Tashiro, K., Nakashima, N., Nishimoto, T., Sekiguchi, T. (2005). Association of the GTP-Binding Protein Gtr1p With Rpc19p, a Shared Subunit of RNA Polymerase I and III in Yeast Saccharomyces cerevisiae. Genetics 170: 1515-1524 [Abstract] [Full Text]  
• Berlyn, M. K. B. (1998). Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map. Microbiol. Mol. Biol. Rev. 62: 814-984 [Abstract] [Full Text]