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Cell Wall Turnover at the Hemispherical Caps of Bacillus subtilis

¹ Department of Genetics and Cell Biology, University of Minnesota, St. Paul, Minnesota 55101

ABSTRACT

Cell walls made by Bacillus subtilis bacteria grown in D₂O medium have buoyant densities in CsCl which are different from walls made by cells grown in H₂O medium. Cell wall turnover was studied by measuring the change in wall buoyant density after a B. subtilis culture was shifted from growth in D₂O medium to aeration in H₂O medium. Walls from the hemispherical caps were isolated after preferential digestion of wall from the cylindrical regions using the B. subtilis autolytic amidase. The walls from the polar regions were found to turn over extensively.