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Use of Ultraviolet Radiation to Locate Dipicolinic Acid in Bacillus cereus Spores

^a Commonwealth Scientific and Industrial Research Organization, Division of Food Research, North Ryde, New South Wales 2113, Australia

ABSTRACT

A novel method is described that allows a direct determination of the location of a portion of the dipicolinic acid (DPA) in spores. Ultraviolet (UV) irradiation is shown to cause cross-linkage of DPA to spore proteins which have the characteristics of membrane proteins. We suggest that DPA resides in the inner forespore membrane (IFSM) and spore cytoplasm (i.e., the spore protoplast). Only that portion of the DPA in the vicinity of the IFSM appeared to form UV-induced DPA-protein adducts.

¹ Present address: School of Dentistry, University of Minnesota, Minneapolis, Minn. 55455.