This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Mann, M. B. Articles by Huang, P. C. Search for Related Content PubMed PubMed Citation Articles by Mann, M. B. Articles by Huang, P. C.

New Chromatographic Form of Phenylalanine Transfer Ribonucleic Acid from Escherichia coli Growing Exponentially in a Low-Phosphate Medium

^a Department of Biochemistry, The Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland 21205

ABSTRACT

Reversed-phase chromatography has been used to detect the presence of a new form of phenylalanyl-transfer ribonucleic acid (Phe-tRNA) from Escherichia coli growing exponentially in media containing low but nonlimiting levels of inorganic phosphate. The amount of this extra Phe-tRNA form is greatest in slowly growing cells (0.8 generations/h), and becomes negligible in media supporting a rapid growth rate (2.14 generations/h).

¹ Present address: Department of Microbiology, The Johns Hopkins University, School of Medicine, Baltimore, Md. 21205.