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Uptake of Nitrite by Neurospora crassa

^a Department of Biology, University of Virginia, Charlottesville, Virginia 22901

ABSTRACT

Like the nitrate transport system, the nitrite uptake system in Neurospora crassa is induced by either nitrate or nitrite. This induction is prevented by cycloheximide, puromycin, or 6-methyl purine. The K_m for nitrite of the induced nitrite uptake system is 86 µM, and the V_max is 100 µmol of nitrite per g (wet weight) per h. Nitrite uptake is inhibited by metabolic poisons such as arsenate, dinitrophenol, cyanide, and antimycin A. No repression or inhibition of the nitrite transport system by ammonia, nitrate, or Casamino Acids was observed.

¹ Present address: Department of Microbiology, University of Virginia, Charlottesville, Va. 22901.

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