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Bioassay for the Isolation of Dictyostelium discoideum Mutants Deficient in Extracellular Accumulation of Cyclic Adenosine 3',5'-Monophosphate

^a Department of Biology, Brandeis University, Waltham, Massachusetts 02154

ABSTRACT

The growth response to external cyclic adenosine 3',5'-monophosphate of a strain of Escherichia coli deleted for adenyl cyclase was utilized to screen for mutants of Dictyostelium discoideum unable to accumulate this chemical extracellularly. The threshold amount of cyclic adenosine 3',5'-monophosphate able to induce growth of this bacterium was 3 to 4 µg/ml at 37 C and approximately 25 µg/ml at 27 C. Conditions are described that permit the detection of as low as 2 µg of this chemical at either temperature.

¹ Present address: Department of Molecular Biology, Hebrew University, Jerusalem, Israel.