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Electron Microscope Analysis of Partial Denaturation of F Factor Deoxyribonucleic Acid

^a Department of Chemistry, California Institute of Technology, Pasadena, California 91109

ABSTRACT

Partial denaturation pattern of sex factor deoxyribonucleic acid of Escherichia coli was studied by electron microscopy. Clustering of the adenine-plusthymine-rich regions in one part of the molecule was revealed. The positions of these regions were located on the physical map of F by analyzing the partial denaturation pattern of heteroduplexes between F and F-prime factors with various parts of F sequences deleted.

¹ Present address: Department of Biochemistry, Stanford University School of Medicine, Stanford, Calif. 94305.