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Negative Chemotaxis in Escherichia coli

Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706
Department of Genetics, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706

ABSTRACT

Several methods for detecting or measuring negative chemotaxis are described. Using these, we have surveyed a number of chemicals for their ability to repel Escherichia coli. Although most of the repellents are harmful compounds, harmfulness is neither necessary nor sufficient to make a compound a repellent. The repellents can be grouped into at least nine classes according to (i) competition experiments, (ii) mutants lacking certain of the negative taxes, and (iii) their chemical structure. The specificity of each class was studied. It is suggested that each class corresponds to a distinct chemoreceptor. Generally, non-chemotactic mutants lack both positive and negative chemotaxis, and L-methionine is required for both kinds of taxis. Repellents at very low concentrations are not attractants, and attractants at very high concentrations are not repellents.

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• Shi, W., Yang, Z., Geng, Y., Wolinsky, L. E., Lovett, M. A. (1998). Chemotaxis in Borrelia burgdorferi. J. Bacteriol. 180: 231-235 [Abstract] [Full Text]  
• Muskavitch, M., Kort, E., Springer, M., Goy, M., Adler, J (1978). Attraction by repellents: an error in sensory information processing by bacterial mutants. Science 201: 63-65 [Abstract]  
• CURRIER, W. W., STROBEL, G. A. (1977). Chemotaxis of Rhizobium spp. to a Glycoprotein Produced by Birdsfoot Trefoil Roots. Science 196: 434-436 [Abstract]  
• Kung, C, Chang, S., Satow, Y, Houten, J., Hansma, H (1975). Genetic dissection of behavior in paramecium. Science 188: 898-904