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Mapping of the Locus for Escherichia coli Transfer Ribonucleic Acid Nucleotidyltransferase

Departments of Microbiology and Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032

ABSTRACT

cca Mutants of Escherichia coli K12 have altered levels of tRNA nucleotidyl-transferase activity. The cca locus has been located at minute 59.4 of the E. coli linkage map. It is cotransduced with tolC but not with argG, and is the earliest known marker transferred by Hfr strain KL14. The proximity of the tolC locus to the integrated sex factor in Hfr strain KL14 may be useful for mapping sex factor mutations by transduction.