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J Bacteriol. 1974 June; 118(3): 783-789
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Mutants of Escherichia coli B/r Defective in Deoxyribonucleic Acid Initiation: dnaI, a New Gene for Replication

^a Max Planck Institut für Molekulare Genetik, Berlin, West Germany

ABSTRACT

Mutagenized E. coli B/r cells were subjected to a procedure designed to select mutants temperature-sensitive for initiation of deoxyribonucleic acid (DNA) replication. Seventeen mutants exhibiting limited residual DNA synthesis at 42 C were obtained and the dna^– sites were mapped genetically. Sixteen of the sites map near dnaA, dnaB, and dnaC. One mutant (dna-208) maps in a new location between the trp and his genes. We propose to call this mutant dnaI208. In complementation experiments dnaC⁺ and dnaI⁺ were dominant to dnaC^– and dnaI^– alleles, respectively. However, dnaA^– was dominant to the wild-type allele dnaA⁺. All dnaA mutants and four out of six dnaC mutants could be suppressed by F factor integration. The pattern of suppression was specific for each mutant.

¹ Present address: Universität Bremen, Bremen, West Germany.

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