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J Bacteriol. 1974 June; 118(3): 796-804
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Purification and Properties of Dextransucrase and Invertase from Streptococcus mutans

Kazuhiro Fukui, Yoshio Fukui and Takafumi Moriyama

Department of Microbiology, Hiroshima University School of Dentistry, Hiroshima 734, Japan

ABSTRACT

Invertase (ß-D-fructofuranoside fructohydrolase, EC 3.2.1.26) and dextransucrase ({alpha}-1, 6-glucan: D-fructose 2-glucosyltransferase, EC 2.4.1.5) were purified from the culture fluids of Streptococcus mutans by chromatography on Sepharose 6B and diethylaminoethyl-cellulose followed by treatment with hydroxyapatite. Each of the enzyme preparations gave a single band when analyzed by either polyacrylamide gel electrophoresis or immunodiffusion. The antigenic determinant of invertase was different from that of dextransucrase on immunodiffusion. The pH optima were 5.25 for invertase and 5.75 for dextransucrase, and the Km values were 20 mM for invertase and 2.0 mM for dextransucrase. The molecular weights determined by sodium dodecyl sulfate gel electrophoresis were 160,000 for invertase and 170,000 for dextransucrase. The data obtained suggest that the dextransucrase had dextran-synthesizing activity and invertase-like activity.

J Bacteriol. 1974 June; 118(3): 796-804
Copyright © 1974 American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Harper, D.S., Loesche, W.J. (1983). Basic Biological Sciences: Effect of pH Upon Sucrose and Glucose Catabolism by the Various Genogroups of Streptococcus mutans. JDR 62: 526-531 [Abstract]  
  • Fukushima, K., Motoda, R., Ikeda, T. (1981). Effects of Exogenous Insoluble Glucan Primer on Insoluble Glucan Synthesis by Streptococcus mutans. JDR 60: 1707-1712 [Abstract]  
  • Bozzola, J. J., Johnson, M. C., Shechmeister, I.L. (1980). Comparison of the Glucosyltransferases of Streptococcus mutans GS-5 to a Non-cariogenic Mutant. JDR 59: 1613-1619 [Abstract]  
  • Montville, T. J., Cooney, C. L., Sinskey, A. J. (1977). Measurement and Synthesis of Insoluble and Soluble Dextran by Streptococcus mutans. JDR 56: 983-989 [Abstract]  
  • Chludzinski, A. M., Germaine, G. R., Schachtele, C. F. (1976). Streptococcus mutans Dextransucrase: Purification, Properties, and Requirement for Primer Dextran. JDR 55: C75-C86  
  • Ciardi, J.E., Hageage, G.J. JR, Wittenberger, C.L. (1976). Multicomponent Nature of the Glucosyltransferase System of Streptococcus mutans. JDR 55: C87-C96  
  • Smith, D. J., Taubman, M. A. (1976). Immunization Experiments Using the Rodent Caries Model. JDR 55: C193-C205  


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