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J Bacteriol. 1974 June; 118(3): 986-989
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Effect of Molybdenum Starvation and Tungsten on the Synthesis of Nitrogenase Components in Klebsiella pneumoniae

Winston J. Brill, Ann L. Steiner and Vinod K. Shah

Department of Bacteriology, College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706

ABSTRACT

Klebsiella pneumoniae M5a1 grows well in the presence or absence of molybdenum in media containing excess NH4+. However, growth on N2 is completely dependent on the presence of molybdenum in the medium. Tungstate competes with the molybdate requirement during growth on N2. In molybdenum-depleted medium, neither protein component of nitrogenase is active and neither component can be detected antigenically. These data provide evidence that molybdenum is an inducer of nitrogenase synthesis.

J Bacteriol. 1974 June; 118(3): 986-989
Copyright © 1974 American Society for Microbiology. All Rights Reserved.



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