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J Bacteriol. 1974 June; 118(3): 990-998
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Threonine Locus of Escherichia coli K-12: Genetic Structure and Evidence for an Operon

¹ Service de Biochimie Cellulaire, Institut Pasteur, 75015 Paris, France

ABSTRACT

Three genes, thrA, thrB, and thrC, were previously defined and localized in the threonine locus of Escherichia coli K-12. thrA, thrB, and thrC specify the enzymes aspartokinase I-homoserine dehydrogenase I, homoserine kinase, and threonine synthetase, respectively. A complementation analysis of the threonine cluster using derivatives of a lambda phage carrying the threonine genes (dthr_c) demonstrates that: (i) thrB and thrC each consist of a single cistron; and (ii) thrA is composed of two cistrons, thrA₁ and thrA₂, although it specifies a single polypeptide chain. thrA₁ and thrA₂ correspond to aspartokinase I and homoserine dehydrogenase I, respectively. Their relative order is established. The demonstration of polar effects of mutations (nonsense or induced by phage Mu) in thrA and thrB is taken as evidence for the existence of a thrA thrB thrC operon, transcribed in this order.

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