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Genetic Analysis of a Temperature-Resistant Revertant of the Conditional Lethal Escherichia coli Double Mutant polA12 uvrE502

The Gamaleya Institute for Epidemiology and Microbiology AMS USSR, Moscow, USSR
Research Institute of Medical Radiology AMS USSR, Obninsk, Kaluga Region, USSR

ABSTRACT

Conditional lethality of the Escherichia coli polA12 uvrE502 double mutant may be overcome by a mutation that has been termed polA350. The polA350 mutation restored the polymerizing activity of deoxyribonucleic acid polymerase I at 42 C in the polA12 mutant and partially suppressed ultraviolet (UV) and methylmethane sulfonate sensitivities of the polA12. Mapping experiments have located polA350 between metE and polA12, very close to the latter. The strain carrying polA12 polA350 and recB21 was viable at 42 C. The effects of the recB21 and polA12 polA350 combination on the UV sensitivity were additive. The triple mutant polA12 polA350 uvrE502 was more UV sensitive than the single uvrE502 mutant.