JB Track the topics, authors and articles important to you
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Eidels, L.
Right arrow Articles by Osborn, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Eidels, L.
Right arrow Articles by Osborn, M. J.
J Bacteriol. 1974 July; 119(1): 138-143
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Transport of D-Arabinose-5-Phosphate and D-Sedoheptulose-7-Phosphate by the Hexose Phosphate Transport System of Salmonella typhimurium

Leon Eidels1, Paul D. Rick, Norma P. Stimler and M. J. Osborn

a Department of Microbiology, University of Connecticut Health Center, Farmington, Connecticut 06032

ABSTRACT

D-Arabinose-5-phosphate and D-sedoheptulose-7-phosphate were found to be substrates, although not inducers, of the hexose phosphate transport system of Salmonella typhimurium. Transport of these two sugar phosphates by wild-type strains required preinduction of the hexose phosphate transport system. A mutant of S. typhimurium constitutive for this system also transported D-arabinose-5-phosphate and D-sedoheptulose-7-phosphate in a constitutive fashion. Glucose-6-phosphate was a potent competitor of the transport of both D-arabinose-5-phosphate and D-sedoheptulose-7-phosphate. The Km values for transport of D-glucose-6-phosphate, D-arabinose-5-phosphate, and D-sedoheptulose-7-phosphate were 0.13, 0.32 and 1.61 mM, respectively. The apparent Vmax values for transport of D-glucose-6-phosphate, D-arabinose-5-phosphate, and D-sedoheptulose-7-phosphate were 6.3, 13.2 and 3.0 nmol per min per 5 x 108 bacteria, respectively. D-Ribulose-5-phosphate and D-xylulose-5-phosphate did not inhibit transport of the above substrates, whereas D-ribose-5-phosphate was a weak inhibitor of D-sedoheptulose-7-phosphate transport.

FOOTNOTES

1 Present address: Department of Microbiology, University of Texas Southwestern Medical School, Dallas, Tex. 75235.

J Bacteriol. 1974 July; 119(1): 138-143
Copyright © 1974 American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Appl. Environ. Microbiol. Infect. Immun. Eukaryot. Cell
Mol. Cell. Biol. J. Virol. Microbiol. Mol. Biol. Rev.
ALL ASM JOURNALS

Copyright © 1974 by the American Society for Microbiology. All rights reserved.