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Adenine Nucleotide Changes Associated with the Initiation of Sporulation in Bacillus subtilis

Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706

ABSTRACT

At the end of the exponential growth phase of Bacillus subtilis, there is a decrease in the energy level of the cell, whether expressed as adenosine triphosphate concentration or adenylate energy charge. Phosphate limitation of exponentially growing cells produces a similar decrease in the energy level of the cell, and sporulation is derepressed in the presence of 10 mM glucose. A reduction in the tryptophan concentration of the medium during phosphate limitation of the tryptophan auxotroph B. subtilis 168 prevented the decrease in energy charge. Cells do not sporulate under these conditions. Energy charge values of 0.30 to 0.35 found during sporulation do not lead to cell death.