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Halophilic Nuclease from a Moderately Halophilic Micrococcus varians

^a Noda Institute for Scientific Research, Noda-shi, Chiba-ken, Japan

ABSTRACT

The moderately halophilic bacterium Micrococcus varians, isolated from soy sauce mash, produced extracellular nuclease when cultivated aerobically in media containing 1 to 4 M NaCl or KCl. The enzyme, purified to an electrophoretically homogeneous state, had both ribonuclease and deoxyribonuclease activities. The nuclease had maximal activity in the presence of 2.9 M NaCl or 2.1 M KCl at 40 C. The enzymatic activity was lost by dialysis against low-salt buffer, whereas when the inactivated enzyme was dialyzed against 3.4 M NaCl buffer as much as 77% of the initial activity could be restored.

¹ Present address: Kagoshima University, Faculty of Agriculture, 1946 Uearata-cho, Kagoshima-shi, Japan.

This article has been cited by other articles:

• Ventosa, A., Nieto, J. J., Oren, A. (1998). Biology of Moderately Halophilic Aerobic Bacteria. Microbiol. Mol. Biol. Rev. 62: 504-544 [Abstract] [Full Text]