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Mechanism of Factor Biosynthesis in Saccharomyces cerevisiae

Institute of Physiological Chemistry II, Ruhr University, 463 Bochum, Germany

ABSTRACT

The biosynthesis of factor, a mating-type-specific regulatory oligopeptide which is secreted by Saccharomyces cerevisiae cells of mating type, was studied. In batch cultures only small amounts of the peptide were synthesized during the exponential growth phase. During the stationary phase, factor was produced at a constant rate and accumulated in the culture medium. Inhibition of translation in wild-type cells by cycloheximide, or in mutant strains under conditions which blocked protein or ribonucleic acid (RNA) synthesis completely inhibited the production of factor. These results indicate that the factor is produced by ribosomal translation of a specific messenger RNA and not by an extraribosomal mechanism of peptide synthesis.