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Glycerol Metabolism in Bacillus subtilis: Gene-Enzyme Relationships

¹ Department of Bacteriology, Karolinska Institutet, S 104 01 Stockholm 60, Sweden

ABSTRACT

Bacillus subtilis mutants unable to catabolize glycerol (Glp mutants) were isolated and mapped. The location of the mutations on the chromosome was determined by a density transfer technique and confirmed by PBS1 transduction and transformation. The different mutations were ordered relative to each other. Mutations rendering the cells glycerol auxotrophic were also mapped and found not to be linked to the Glp mutations.

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