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Bacterial Cell Division Regulation: Characterization of the dnaH Locus of Escherichia coli

¹ Department of Microbiology, The University of Texas at Austin, Austin, Texas 78712

ABSTRACT

The dnaH locus is the fourth gene to be identified as required for deoxyribonucleic acid polymerization in Escherichia coli. A temperature-sensitive mutant defective in this gene exhibited an abrupt decrease in rate of deoxyribonucleic acid synthesis when shifted to 42 C. The locus mapped in the proC-purE region of the chromosome by conjugation and was co-transducible with purE. dnaH⁺ is carried on the F'₁₃ episome and is dominant over the dnaH^– mutation.

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