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Mapping of a Mutation, polB100, Affecting Deoxyribonucleic Acid Polymerase II in Escherichia coli K-12

^a Department of Biological Chemistry, Harvard Medical School, Boston, Massachusetts 02115

ABSTRACT

Direct assay for deoxyribonucleic acid polymerase II in extracts has been used to screen recombinants for the polB allele in Hfr x F^– crosses, F-ductants in episome transfer, and transductants in generalized transduction by phage P1. The polB gene is located at 2 min on the Escherichia coli linkage map; it is 39 to 64% co-transducible with leu. A mutant, E. coli polA1 polB100 polC (ts), deficient in deoxyribonucleic acid polymerases I and II and having a thermolabile deoxyribonucleic acid polymerase III, has been prepared by the P1-mediated cross: P1 (HMS85 polB100) x E. coli BT1026 polA1 polC (ts) leu^–.

¹ Present address: Department of Biochemistry, Baylor College of Medicine, Houston, Tex. 77025.

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