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Lipophilic Proteins of Mitochondria from Microaerobic and Aerobic Continuous Cultures of Saccharomyces cerevisiae

¹ Department of Developmental Biology, Research School of Biological Sciences, Australian National University, Canberra, A.C.T., 2601, Australia
² Department of Biochemistry, School of General Studies, Australian National University, Canberra, A.C.T., 2600, Australia

ABSTRACT

Products of the mitochondrial protein-synthesizing system have been labeled in vivo in the presence of cycloheximide in microaerobic cells and in cells from glucose-limited and glucose-repressed aerobic continuous cultures of Saccharomyces cerevisiae. Lipophilic proteins were extracted from labeled mitochondrial membranes with aqueous methanol and neutral and acidic chloroform-methanol solvents. In glucose-limited aerobic and microaerobic cells, about half of the total mitochondrial products were soluble in organic solvents; in contrast, almost all of the labeled products were extracted from glucose-repressed mitochondria. Only trace amounts of labeled product were formed in mitochondrial membranes of a petite mutant. Lipophilic proteins were examined by polyacrylamide gel electrophoresis under dissociating conditions. Most of the label was associated with components of apparent molecular weights 12,000, 14,000 and 16,000. The relative proportions of these species in mitochondrial membranes are dependent on the concentrations of oxygen and glucose in which the cells are grown.