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Con^– Mutants: Class of Mutants in Escherichia coli K-12 Lacking a Major Cell Wall Protein and Defective in Conjugation and Adsorption of a Bacteriophage

¹ Department of Microbiology, University of Adelaide, Adelaide, South Australia 5001

ABSTRACT

We describe a new class of mutants of Escherichia coli K-12 defective in conjugation (Con^– mutants). They lack a major protein of the outer membrane and are defective as recipients with Hfr and F' donors and as recipients for several F-like R factors and Col factors. In the case of crosses with an Hfr donor, we have shown that the Con^– recipient is defective in pair formation. The mutants are resistant to certain phages due to loss of receptor activity.

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