This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kessin, R. H. Articles by Newell, P. C. Search for Related Content PubMed PubMed Citation Articles by Kessin, R. H. Articles by Newell, P. C.

Linkage Analysis in Dictyostelium discoideum Using Temperature-Sensitive Growth Mutants Selected with Bromodeoxyuridine

^a Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom

ABSTRACT

Amoebae of the cellular slime mold Dictyostelium discoideum grown in the presence of bromodeoxyuridine are killed on exposure to near-ultraviolet light. By using this phenomenon, a method was devised by which mutants of D. discoideum that are temperature-sensitive for growth can be readily obtained. Three such mutants have been characterized genetically and each was found to be associated with a different linkage group. Two of these linkage groups have not previously been described.

¹ Present address: The Biological Laboratories, Harvard University, Cambridge, Mass. 02138.

This article has been cited by other articles:

• Konfortov, B. A., Cohen, H. M., Bankier, A. T., Dear, P. H. (2000). A High-Resolution HAPPY Map of Dictyostelium discoideum Chromosome 6. Genome Res. 10: 1737-1742 [Abstract] [Full Text]