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Bacterial Calcium Transport: Energy-Dependent Calcium Uptake by Membrane Vesicles from Bacillus megaterium

Department of Oral Biology, School of Dental Medicine, The University of Connecticut Health Center, Farmington, Connecticut 06032

ABSTRACT

Membrane vesicles capable of energy-dependent calcium uptake have been prepared from Bacillus megaterium cells in log-phase growth or when undergoing sporulation. The uptake is dependent on the calcium concentration and appears saturable in vesicles from cells in log-phase growth. Both ascorbate and phenazine methosulfate are needed as a source of electrons for the energy-dependent increase in calcium uptake. Addition of 8 mM sodium cyanide inhibited the energy-dependent uptake. If this calcium uptake mechanism is a component of the sporulation-specific calcium accumulation process, the latter's functional expression would appear to be inhibited during log-phase growth.

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