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Genetic Location of Two Mutations Affecting the Lysyl-Transfer Ribonucleic Acid Synthetase of Bacillus subtilis

^a Laboratory of Developmental Microbiology, Department of Microbiology, University of Virginia Medical School, Charlottesville, Virginia 22901

ABSTRACT

Two mutations (lysS1 and lysS2), each independently resulting in a thermosensitive, lysyl-transfer RNA synthetase (L-lysine: tRNA ligase [adenosine 5'-monophosphate] EC 6.1.1.6), have been mapped on the Bacillus subtilis chromosome between purA16 (adenine requirement) and sul (sulfanilamide resistance). They are linked by transformation with sul (70 to 74% cotransfer) in the order purA16-lysS1-lysS2-sul. The mutant loci are either in the same gene or in two closely linked genes. They are not linked to the tryptophanyl-tRNA synthetase structural gene or to the lys-1 locus.

¹ Present address: Department of Biological Chemistry, University of Illinois Medical Center, Chicago, Ill. 60612.