This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lloyd, R. G. Articles by Birge, E. A. Search for Related Content PubMed PubMed Citation Articles by Lloyd, R. G. Articles by Birge, E. A.

Isolation and Characterization of an Escherichia coli K-12 Mutant with a Temperature-Sensitive RecA^– Phenotype

^a Yale University School of Medicine, Radiobiology Laboratories, New Haven, Connecticut 06510

ABSTRACT

A mutation that causes a temperature-sensitive RecA^– phenotype was identified in a derivative of a PolA^– strain that failed to grow at high temperature. The mutant allele (recA200) was shown to be linked to cysC, conferred a sharply temperature-sensitive, ultraviolet-sensitive Rec^– phenotype in the range 35 to 42 C, and in crosses failed to show complementation at 42 C with Hfr's that transferred recA^–. Double mutants that carried both recA200 and polA were examined for ability to grow and synthesize DNA at restrictive temperatures.

¹ Present address: Department of Genetics, University of Nottingham, School of Biological Sciences, University Park, Nottingham NG7 2RD, Great Britain.

² Present address: Department of Botany and Microbiology, Arizona State University, Tempe, Ariz. 85281.

This article has been cited by other articles:

• Mahdi, A. A., Buckman, C., Harris, L., Lloyd, R. G. (2006). Rep and PriA helicase activities prevent RecA from provoking unnecessary recombination during replication fork repair. Genes Dev. 20: 2135-2147 [Abstract] [Full Text]  
• Maisnier-Patin, S., Nordstrom, K., Dasgupta, S. (2001). RecA-Mediated Rescue of Escherichia coli Strains with Replication Forks Arrested at the Terminus. J. Bacteriol. 183: 6065-6073 [Abstract] [Full Text]  
• Kuzminov, A. (1999). Recombinational Repair of DNA Damage in Escherichia coli and Bacteriophage lambda. Microbiol. Mol. Biol. Rev. 63: 751-813 [Abstract] [Full Text]  
• Mueller, J E, Clyman, J, Huang, Y J, Parker, M M, Belfort, M (1996). Intron mobility in phage T4 occurs in the context of recombination-dependent DNA replication by way of multiple pathways.. Genes Dev. 10: 351-364 [Abstract]