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Partial Purification of a Membrane-Associated Deoxyribonucleic Acid Complex from Mycoplasma gallisepticum

^a Departments of Microbiology and Radiation Biology and Biophysics, University of Rochester, School of Medicine and Dentistry, Rochester, New York 14642

ABSTRACT

A Mycoplasma gallisepticum subcellular fraction (P2), which contains the deoxyribonucleic acid replication complex, can be isolated by differential centrifugation of freeze-thaw-lysed cells. The nascent deoxyribonucleic acid is released from P2 by Lubrol-WX, sodium dodecyl sulfate, Pronase, and deoxyribonuclease, but not by saponin, ribonuclease, phospholipase C, or high-frequency sonic treatment. Sonic treatment further fractionates the cell ghost and allows partial purification, on sucrose density gradients, of a deoxyribonucleic acid replication complex attached to the cells' polar membrane-bleb-infrableb structures.

¹ Present address: Worchester Foundation for Experimental Biology, Shrewsbury, Mass., 01545.

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