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Properties of the Relaxation Complexes of Supercoiled Deoxyribonucleic Acid and Protein of the R Plasmids R64, R28K, and R6K

^a Department of Biology, University of California at San Diego, La Jolla, California 92037

ABSTRACT

The presence of supercoiled deoxyribonucleic acid in the form of a relaxation complex is described for the antibiotic resistance plasmids R64, R28K, and R6K. The properties of these relaxation complexes indicate that they consist of a covalently closed circular deoxyribonucleic acid molecule associated with an activable, single strand-specific endonuclease.

¹ Present address: Departamento de Genetica, Centro de Investigacion, Apartado Postal 14-740, Mexico 14 D. F., Mexico.

² Present address: Research School of Biological Sciences, The Australian National University, Canberra City, Australia.