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Properties of Thermosensitive Extracellular -Amylases of Bacillus subtilis

Institute of Applied Microbiology, The University of Tokyo, Bunkyo-ku, Tokyo, Japan

ABSTRACT

Enzymological properties of four thermosensitive -amylases (M3, M9, M18, and M20) brought by different mutation sites in -amylase structural gene of Bacillus subtilis were compared with those of the parental -amylase NA64. Two thermosensitive -amylases (M9 and M20) were altered not only in their thermosensitivity but also in their immunological properties, catalytic properties, molecular weights determined by the gel filtration on a Bio-Gel P-100 column, and others. The other two thermosensitive -amylases (M3 and M18) were altered only in their thermosensitivity.