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Nature of Ribonucleic Acid Synthesis During Early Sporulation in Saccharomyces cerevisiae

^a Rosenstiel Basic Medical Sciences Research Center, and Department of Biology, Brandeis University, Waltham, Massachusetts 02154

ABSTRACT

Phosphate uptake in sporulating cultures of Saccharomyces cerevisiae has been found to occur approximately 2 h after the transfer to sporulation medium. Early ribonucleic acid synthesis begins at approximately 4 h and continues to 8 h. Incorporation of phosphate into acid-extractable precursor pools parallels phosphate uptake. In triple-labeling experiments it was observed that the breakdown of vegetatively synthesized ribonucleic acid is not a significant source of precursors for ribonucleic acid synthesis during sporulation. The majority of the ribonucleic acid made in a 10-min period during sporulation does not migrate on gels with precursor or mature ribosomal ribonucleic acid.

¹ Present address: Department of Biology, Texas Christian University, Fort Worth, Tex. 76129.