This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Taber, H. Articles by Freese, E. Search for Related Content PubMed PubMed Citation Articles by Taber, H. Articles by Freese, E.

Sporulation Properties of Cytochrome a-Deficient Mutants of Bacillus subtilis

¹ Department of Microbiology, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642
² Laboratory of Molecular Biology, National Institute of Neurological Diseases and Stroke, National Institutes of Health, Bethesda, Maryland 20014

ABSTRACT

Three classes of cytochrome a-deficient mutants of Bacillus subtilis have been found to be asporogenic or oligosporogenic. All three classes showed declines in adenosine 5'-triphosphate (ATP) concentrations during early sporulation, at a time when ATP levels in wild-type strains are constant. Class III mutants were found to be deficient in aconitase and isocitric dehydrogenase, and showed reduced maximum growth in nutrient sporulation medium. These mutants also suffered the most rapid decline in ATP concentration in early sporulation, and exhibited neither the biphasic oxygen consumption curve nor the increase in pH normally observed at the end of logarithmic growth in nutrient sporulation medium. Nicotinamide adenine dinucleotide oxidase activities of purified membrane preparations were approximately normal for mutants in all classes, except for two of the class II mutants and one class III mutant. Neither cytochrome a nor cytochrome c appears to be an obligatory intermediate in cyanide-sensitive nicotinamide adenine dinucleotide oxidation in B. subtilis.

This article has been cited by other articles:

• Day, W. A. Jr., Rasmussen, S. L., Carpenter, B. M., Peterson, S. N., Friedlander, A. M. (2007). Microarray Analysis of Transposon Insertion Mutations in Bacillus anthracis: Global Identification of Genes Required for Sporulation and Germination. J. Bacteriol. 189: 3296-3301 [Abstract] [Full Text]