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J Bacteriol. 1974 December; 120(3): 1364-1369
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Molecular Nature of the Deoxyribonucleic Acid of a Thermosensitive R Factor, Rts1

¹ Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19174

ABSTRACT

The deoxyribonucleic acid of the thermosensitive R factor, Rts1, has been examined by the technique of sedimentation in alkaline sucrose, electron microscopy, and radiation target size. All these methods yielded a molecular weight of approximately 120 million for Rts1 deoxyribonucleic acid in Escherichia coli. Sedimentation analysis revealed that Rts1 deoxyribonucleic acid in Proteus mirabilis was also 120 million daltons. Rts1 did not segregate into E. coli minicells under the conditions where another smaller non-thermosensitive R factor could.